ZIS LSM 510 META (level 3)

** This microscope is kindly housed within QBI by Zeiss as part of their imaging facility on level 3**

Inverted point-scanning laser confocal microscope with spectral detection. Ideal for imaging live cells or tissue.

Features Include:

  • CO2 and temperature incubation for live cells and tissue
  • Fully motorised X-Y-Z stage
  • Z-stacks
  • Tiled imaging
  • Mark and Find
  • Automated image capture experiments
  • Online fingerprinting – for imaging with multiple spectrally overlapping fluorophores and removing background fluorescence

Objectives:

  • Air: 5x (0.15), 10x (0.3) , 20x (0.8)
  • Oil: 40x (1.4), 63x (1.4), 100x (1.4)

Fluorescent Filter Sets (for checking sample with oculars):

  • DAPI
  • GFP / Alexa 488
  • Cy3 / Alexa 546/555/568
  • Cy5 / Alexa 633/647

Lasers:

  • Blue laser diode (405nm)
    • DAPI
  • Argon laser (458/477/488/514nm)
    • GFP / YFP / FITC / Alexa488
  • HeNe 561nm
    • Cy3 / Alexa 546/555/568 / Alexa 594 / mCherry
  • HeNe 633nm
    • Cy5 / Alexa647

Instructions

Guide to using the LSM 510 META