QBI Histology and Microscopy

Queensland Brain Institute's Advanced Microimaging and Analysis Facility

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    • The QBI Histology Facility
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      • *download complete guide as a pdf document
  • Instruments
    • Confocal Microscopes
      • LSM 510 META Violet (610)
      • ZIS LSM 510 META (level 3)
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      • Fixation – Using Paraformaldehyde
      • Animal Trans-cardial Perfusion Technique
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    • Recipes and Solutions
      • Mowiol
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      • Marinas TIRF Microscope Guide
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      • Stereology Microscope Guide
      • Stereology Information Handout
      • Stereology: 2D Coverslip Cell Counts, Neurite Lengths and Branching
    • Slide Scanning
      • Slide Scanning Information Handout
      • Bright Field Whole Slide Scanning Workflow
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      • Cropping tissue sections from slide scanner images
  • Image Analysis
    • Neurolucida: Automated Analysis of Scanned Slides
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      • Imaris Workstation 1 (level 4)
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      • Single Molecule Localisation Techniques (PALM/STORM)
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      • Super Resolution Articles
      • Fluorophores for PALM and STORM
      • PALM/STORM Image Reconstruction
    • TIRF (total internal reflection fluorescence)
    • FRET (Förster resonance energy transfer)
    • FRAP (Fluorescence recovery after photobleaching)
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Sodium Borohydride

Place sections in 1% sodium borohydride in PBS for 20 minutes.

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