QBI Histology and Microscopy

Queensland Brain Institute's Advanced Microimaging and Analysis Facility

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  • Home
  • About
    • The QBI Microscopy Facility
    • The QBI Histology Facility
    • QBI Guide to Histology and Microscopy
      • *download complete guide as a pdf document
  • Instruments
    • Confocal Microscopes
      • LSM 510 META Violet (610)
      • ZIS LSM 510 META (level 3)
      • LSM 5 LIVE (412)
      • LSM 5 PASCAL (618)
    • Live Imaging Microscopes
      • Marinas TIRF FRET (618)
      • Axio Observer Indigo (618)
      • ZIS LSM 510 META (level 3)
      • LSM 5 LIVE (412)
    • Slide Scanners
      • Bright Field Slide Scanner (414)
      • Fluorescent Slide Scanner (414)
    • Stereology Instruments
      • Stereology Microscope (618)
      • Stereology Workstation (level 4)
    • Upright Microscopes
      • AxioPlan 2 Purple (412)
      • Axio Imager Orange (319)
      • Axio Imager Azure (414)
      • Axio Imager Green (414)
      • Axio Imager Blue (618)
    • Inverted Microscopes
      • Axio Observer Yellow (319)
      • Axio Observer Red (412)
      • Axio Observer Indigo (618)
      • Bob Olympus Inverted (610)
      • Marinas TIRF FRET (618)
    • Stereo Microscopes
      • StereoDiscovery (618)
      • Olympus Stereoscope (411)
  • Bright Field Imaging
    • Planning Your Experiment
    • Preparing Your Sample
      • Fixation – Using Paraformaldehyde
      • Animal Trans-cardial Perfusion Technique
      • Sectioning Your Tissue
      • Tissue Preparation for Vibratome Sectioning
      • Antigen Retrieval
    • Bright Field Protocols
      • Cresyl Violet Staining (Nissl Staining)
      • DAB Staining
      • Nickel DAB (N-DAB) Labeling
      • Haemotoylin and Eosin Staining
      • Neuron Recovery of Neurobiotin/biocytin labeled cells
      • Luxol Fast Blue
  • Fluorescence Imaging
    • Planning Your Experiment
    • Preparing Your Sample
      • Fixation – Using Paraformaldehyde
      • Animal Trans-cardial Perfusion Technique
      • Sectioning Your Tissue
      • Tissue Preparation for Vibratome Sectioning
      • Antigen Retrieval
    • Labeling Your Sample
      • Immunohistochemistry
      • Fluorescence and Chromogenic Labeling
      • Fluorescent Marker Guide
    • Fluorescent Marker Guide
    • Fluorescence Protocols
      • Immunohistochemistry (cultured cells and monolayers)
      • Immunohistochemistry (Tissue Sections)
      • Immunohistochemistry with Tyramide Signal Amplification (TSA)
    • Recipes and Solutions
      • Mowiol
      • Chrome Alum Gelatin Slide Coating
      • 8% Paraformaldehyde for Immunofluorescence
    • Imaging Your Sample
      • Choosing the right technique
      • Exposure times
      • Fluorescent crosstalk / bleed-through
      • Autofluorescence
      • Keep things consistent
  • Instructions and Guides
    • Standard Microscopes
      • Axio Imager / Observer Guide
      • StereoDiscovery (Magenta) Guide
      • AxioPlan 2 Guide
      • Bright Field Imaging Guide
      • Guide to using ApoTome
      • High-Throughput Imaging Guide
    • Confocal Microscopes
      • LSM 5 PASCAL Guide
      • LSM 510 META Confocal Guide
    • Marinas TIRF FRET FLIM Microscope
      • Marinas TIRF Microscope Guide
    • Stereology
      • Stereology Microscope Guide
      • Stereology Information Handout
      • Stereology: 2D Coverslip Cell Counts, Neurite Lengths and Branching
    • Slide Scanning
      • Slide Scanning Information Handout
      • Bright Field Whole Slide Scanning Workflow
      • Bright Field Tissue Segmentation Workflow
      • Bright Field Manual Scanning Workflow
      • Cropping tissue sections from slide scanner images
  • Image Analysis
    • Neurolucida: Automated Analysis of Scanned Slides
    • Imaris Guides
      • Imaris: Creating a Surface
      • Imaris : Creating Cell Surfaces on Undefined Cells
      • Imaris: Using a surface to mask a channel
      • Imaris: Counting Cells on Slide Scanner Images
      • Imaris: Neuron Tracing with the Filament Tracing Wizard
      • Colocalisation
    • Analysis Workstations
      • Imaris Workstation 1 (level 4)
      • Imaris Workstation 2 (level 4)
      • Imaris Workstation 3 (level 4)
      • Stereology Workstation (level 4)
      • AxioVision Workstation (level 6)
    • Analysis Software
      • Image J / FIJI
      • Imaris
      • SlideCrop
      • Stereo Investigator
      • Neurolucida
      • Vaa3D
  • Advanced Techniques
    • Super Resolution Microscopy
      • Single Molecule Localisation Techniques (PALM/STORM)
      • Structured Illumination Microscopy (SIM)
      • Photobleaching with non-linear processing (PiMP)
      • Super Resolution Optical Fluctuation Microscopy (SOFI)
      • Super Resolution Articles
      • Fluorophores for PALM and STORM
      • PALM/STORM Image Reconstruction
    • TIRF (total internal reflection fluorescence)
    • FRET (Förster resonance energy transfer)
    • FRAP (Fluorescence recovery after photobleaching)
    • Stereology
    • Slide Scanning
    • Live Imaging
  • Images and Movies
    • QBI Art in Neuroscience
      • 2008 Competition
      • 2009 Competition
      • 2010 Competition
      • 2011 Competition
  • Links
  • Request Help / Training
  • Book an Instrument

High-Throughput Imaging Guide

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