There are still 7 places left in the 2013 Imaris Training Workshop hosted by Institute for Molecular Bioscience and the Queensland Brain Institute. The workshop was only announced yesterday so these places will be filled in a few hours.
Please RSVP to Toula Georgiou (firstname.lastname@example.org) as soon as possible to ensure your place in the program.
The 2-day workshop will provide hands-on training for using many of the advanced features of Imaris. The course is provided at no cost to the participants and is open to everyone. However, space is strictly limited to forty participants.
An example of the power of Imaris based image analysis can be seen in our recent publication found in Frontiers in Neuronal Circuits where 3D neuron reconstructions were used to localise pre- and post-synpatic proteins. This technique provides an ability to easily gauge the number of synaptic inputs on a neuron.
You can read the full article here.
Studying cellular morpho-dynamics via bioimage informatics
Dr. Alexandre Dufour
Tuesday 10 September 2013 at 1pm – Auditorium, QIMR Central
Cell deformation and migration are key factors involved in numerous aspects of cell development, immune responses, cancer and infectious diseases. Dr Alexandre Dufour’s team develops image analysis tools and software to extract quantitative information from multi-dimensional, multi-modal live microscopy, with the aim to derive mathematical models of cellular morpho-dynamics.
To this end, his team develops a repertoire of computational approaches to
- automatically track multiple cells in 2D/3D microscopy in cluttered environments and low signal-to-noise conditions,
- efficiently describe the 3D morphology of cells despite their natural shape variability, and
- extract biophysical quantities at the intra-cellular level directly from imaging data alone.
His team is also actively involved in the development of the open-source Icy platform, which enables simple and fast dissemination of such tools to the scientific community.
On Friday the 13th of September QBI will be hosting the Zeiss User Group Meeting in the level 7 Auditorium.
3:00 – 3:45pm Dr. Mark Stafford from Zeiss will discuss advancements in correlative light and electron microscopy and the application of light sheet imaging.
3:45 – 4:30pm Luke Hammond will introduce the advanced imaging suite at QBI and discuss how super-resolution techniques along with new scanning and spinning disk confocal instruments will impact research at QBI.
Please join us on the terrace afterwards for wine and cheese kindly provided by Zeiss.
Initial training for the Zeiss Elyra super-resolution instrument will be taking place on Monday and Tuesday (23 and 24th of September) if you are interested in taking part in this first round of training please let me know – email@example.com
For those of you interested in imaging 3D neuronal structure we have been able to use the technique of Spiga et al. (Simultaneous Golgi-Cox and immunofluorescence using confocal microscopy) to image Golgi-Cox stained neurons at high-resolution on the confocal in mouse brain sections. This technique uses laser reflection so can be scanned faster than typical fluorescence signal and is suitable for spine analysis. When larger cell numbers are required for analysis it may be a useful alternative to filling individual neurons.
Steve Lee at ANU Research School of Engineering is experienced in building and developing in vivo imaging probes for cellular imaging of internal organs. Steve is looking for new researchers to collaborate with to build novel imaging systems. If you are interested in using in vivo imaging for your work he may be able to help you – see one of Steve’ recent papers in Nature Protocols here.
Ventana are running a seminar at QBI on Thursday 8th August at 10:30am to discuss their advances in automated tissue and slide staining. If you are interested in attending please RSVP to Janet (firstname.lastname@example.org) with the form below.
Instruments for the ARC-LIEF funded super resolution and advanced imaging facility will begin arriving at QBI in September.
Thanks to the success of the LIEF application lead by Associate Professor Fred Meunier QBI will be receiving an updated version of the Zeiss Elyra PS.1 Super Resolution Microscope (capable of both SIM and single molecule imaging) , a Roper iLas2 Super Resolution Microscope (capable of rapid single molecule imaging) and a Zeiss LSM 710 Inverted Confocal fitted with high sensitivity GaAsP detectors.
For more information on super resolution please see the links above or in the menu under Advanced Techniques.
Jennifer Lippincott-Schwartz has posted a good introduction to super resolution imaging on youtube – available below
If you would like to discuss how super resolution may be used to assist your research or need help planning your experiments to take advantage of these new instruments please contact Dan or myself.
Since the publication of the Clarity tissue clearing method – we have been working to optimise a protocol for researchers here at QBI. Thanks to Jane Ellis and Rob Sullivan we have already cleared initial mouse and zebrafish samples – see an example of the results here – clarity 40xmgp.
SeeDB, another recently published clearing agent, is worth keeping in mind especially for thick sections used for imaging filled neurons. SeeDB does not clear tissue as thoroughly as Clarity but has the advantage of not swelling tissue samples while being faster and far less toxic.
Please get in touch with Jane, Dan or myself if you are interested in using either of these techniques for clearing thick sections or whole tissue.
Lastly, thanks to Dan we now have an updated instruction guide for using Zen Blue 2012 on Axio Imager Blue.
Just a reminder that Geraint Wilde, the Andor Revolution application specialist, will be presenting today at 11am in the QBI Auditorium on the Andor revolution spinning disk instrument.
Also, tomorrow, Tuesday May 21st, Isuru Jayasinghe will be presenting his latest research using super resolution microscopy. Room 305 Skerman @ 12 noon.
Andor are bringing their Revolution spinning disk instrument to QBI for demonstration on Monday 20th to Thursday 30th of May.
Geraint Wilde, the Andor Revolution application specialist, will be presenting on Monday 20th at 11am in the QBI Auditorium to introduce the capabilties of this instrument. The Andor revolution instrument is designed for very high speed confocal imaging with low phototoxicity – especially suited to:
- live cell dynamics and protein trafficking
- calcium imaging
- imaging model organisms
- rapid imaging of fixed and live samples such as 3D mosaics
To organise time on the instrument while it is here please contact email@example.com
3i Spinning Disk April Demonstration
Special thanks to 3i (intelligent imaging innovations) for bringing out their spinning disk instrument to QBI for demonstration in April. The data generated during this time is now available on the server and includes large tissue montages and overnight 4D imaging of neuronal development.
Zeiss LightSheet Microscope Demonstration
Zeiss are bring their new Lightsheet microscope to UQ next week.
Workshops will take place at The Institute for Molecular Bioscience (IMB), Building 80, The University of Queensland, from 6th – 17th of May. Both morning and afternoon sessions are available. RSVP today to reserve your place.
New Methods Available on the Microscopy Website
Recent Publications from QBI using the Microscopy Facility
Calcagno, B., Eyles, D., van Alphen, B. & van Swinderen, B. (2013) Transient activation of dopaminergic neurons during development modulates visual responsiveness, locomotion and brain activity in a dopamine ontogeny model of schizophrenia. Translational Psychiatry 2: e2026.
Fothergill, T., Donahoo, A.-L. S., Douglass, A., Zalucki, O., Yuan, J., Shu, T., Goodhill, G. J. & Richards, L. J. (2013) Netrin-DCC signaling regulates corpus callosum formation through attraction of pioneering axons and by modulating Slit2-mediated repulsion. Cerebral Cortex [Epub ahead of print].
Kirszenblat, L., Neumann, B., Coakley, S. & Hilliard, M. A. (2012) A dominant mutation in MEC-7/β-tubulin affects axon development and regeneration in C. elegans neurons. Molecular Biology of the Cell [Epub ahead of print].
Kottler, B., Bao, H., Zalucki, O., Imlach, W., Troup, M., van Alphen, B., Paulk, A., Zhang, B. & van Swinderen, B. (2013) A sleep/wake circuit controls isoflurane sensitivity in Drosophila. Current Biology 23(7): 594–598.
Martin, S., Harper, C. B. ˢ., May, L. M., Coulson, E. J., Meunier, F. A. & Osborne, S. L. (2013) Inhibition of PIKfyve by YM-201636 dysregulates autophagy and leads to apoptosis-independent neuronal cell death. PLoS ONE 8(3): e60152.
Yuan, J.,Chan, S., Mortimer, D., Nguyen, H. & Goodhill, G. J. (2013) Optimality and saturation in axonal chemotaxis. Neural Computation 25(4): 833-853.
Vukovic, J., Borlikova, G. G., Ruitenberg, M. J., Robinson, G. J., Sullivan, R. K., Walker, T. L. & Bartlett, P. F. (2013) Immature doublecortin-positive hippocampal neurons are important for learning but not for remembering. Journal of Neuroscience 33(15): 6603-6613.
Prof. Sergio Grinstein and Prof. Amira Klip will be speaking at the Leica Scientific Forum this Friday March 15 at 12:00pm in the QBP Auditorium (IMB).
Please write to firstname.lastname@example.org for free registration to this event.